Humoral immune responses induced by PR8-amiR-93NP and protection against wild PR8 influenza virus in mice. Mice (n=8) were vaccinated with different doses of PR8-amiR-93NP and bled at days 0, 15 and 29 post-vaccination. At 29 days post-vaccination, Mice (n=8) were challenged with 100×LD50.HA-specific antibodies IgG (A), IgG1 (B), and IgG2a (C) were measured by ELISA. (* p>0.05, **p<0.05). (D) Mouse weight change after challenge and (E) survival rate (* p>0.05) are shown.