Skip to main content
. 2015 May 20;10(5):e0124094. doi: 10.1371/journal.pone.0124094

Fig 1. Specific recognition of autologous melanoma cells by CD4+ TIL155 clones.

Fig 1

(A) Specific antitumor recognition of CD4+ TIL155-C1-4. The reactivity of four CD4+ TIL155 T cell clones to autologous 155mel cells, allogeneic melanoma cell lines, and the 586EBV-B cell line was evaluated based on IFN-γ release. (B) HLA restriction of T cell recognition. CD4+ TIL155-C1 cells were co-cultured with autologous 155mel cells in the presence or absence of various anti-MHC antibodies. IFN-γ release was determined after an 18-h incubation. (C) Cytokine profiles, measured by ELISA, of TIL155-C1 and TIL155-C2 cell culture supernatants after 155mel tumor cell stimulation.