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. 2015 May 20;10(5):e0125005. doi: 10.1371/journal.pone.0125005

Table 2. Kinetic parameters for the nitrite reductase activity of the Cr-TrHb1s.

Cr-TrHb1-1 Cr-TrHb1-2 Cr-TrHb1-4
Parameters of the nitrite reductase activity pH 6.6 and 20°C
Fast Process
k -L (s-1) 7.2±1.1 9.3±1.9 2.6(±0.4)×101
ρ 1.0(±0.3)×102 1.4(±0.4)×102 5.7±0.9
Slow Process
h on (M-1 s-1) 9.8(±3.4)×101 5.4(±1.7)×101 6.9±2.4
Parameters fitting the pH-dependence of nitrite reductase activity at 20°C a
f h 0 (s-1) 1.8±0.3 7.0(±1.2)×101 6.6±1.0
f h 1 (s-1) 1.0(±0.2)×101 7.8(±1.3) 3.1(±0.7)×101
s h 0 (s-1) 5.1(±0.8)×10−3 8.6(±2.1)×10−1 1.4(±0.3)×10−2
s h 1 (s-1) 2.2±0.4 - 1.2±0.3
pK af 6.8±0.2 8.0±0.2 7.2±0.2
pK as 5.8±0.2 - 5.4±0.2

a Values obtained at [NO2 -] = 5.0×10–2 M after mixing.