Table 2. Kinetic parameters for the nitrite reductase activity of the Cr-TrHb1s.
Cr-TrHb1-1 | Cr-TrHb1-2 | Cr-TrHb1-4 | |
---|---|---|---|
Parameters of the nitrite reductase activity pH 6.6 and 20°C | |||
Fast Process | |||
k -L (s-1) | 7.2±1.1 | 9.3±1.9 | 2.6(±0.4)×101 |
ρ | 1.0(±0.3)×102 | 1.4(±0.4)×102 | 5.7±0.9 |
Slow Process | |||
h on (M-1 s-1) | 9.8(±3.4)×101 | 5.4(±1.7)×101 | 6.9±2.4 |
Parameters fitting the pH-dependence of nitrite reductase activity at 20°C a | |||
f h 0 (s-1) | 1.8±0.3 | 7.0(±1.2)×101 | 6.6±1.0 |
f h 1 (s-1) | 1.0(±0.2)×101 | 7.8(±1.3) | 3.1(±0.7)×101 |
s h 0 (s-1) | 5.1(±0.8)×10−3 | 8.6(±2.1)×10−1 | 1.4(±0.3)×10−2 |
s h 1 (s-1) | 2.2±0.4 | - | 1.2±0.3 |
pK af | 6.8±0.2 | 8.0±0.2 | 7.2±0.2 |
pK as | 5.8±0.2 | - | 5.4±0.2 |
a Values obtained at [NO2 -] = 5.0×10–2 M after mixing.