Fig 1. Brain amyloid beta 1–40 (Aβ 1–40) levels and Aβ immunoreactivity increased with 9 month airborne particulate matter exposure while no change in oligomerized protein staining was observed.
Brains of 9 month exposed filtered air (FA) or PM2.5 (PM) mice were collected with the left hemisphere fixed for immunohistochemistry and the right temporal cortices collected for biochemical analysis. A. Rodent Aβ 1–40 levels from FA and PM temporal cortices were quantified by ELISA, *p<0.05. B. Temporal cortex lysates from FA and PM brains were used for dot blot analysis using A11 anti-oligomer antibody and α-tubulin (loading control). C. A11 optical densities were normalized to their respective loading control, averaged (+/-SD), and graphed. Brains were immunostained using D, anti-A11 antibody to visualize oligomeric protein, E, anti-rodent specific Aβ antibody, and F, anti-Aβ antibody 4G8. Images shown are representative from the temporal cortex. Arrows indicate regions taken for higher magnification.