Fig 4. Brain beta-site APP cleaving enzyme (BACE) protein levels increased after 9 month exposure to airborne particulate matter.
Brains of 9 month exposed filtered air (FA) or PM2.5 (PM) mice were collected with the left hemisphere fixed for immunohistochemistry and the right temporal cortices collected for biochemical analysis. A. Temporal cortex lysates from FA and PM brains were used for western blot analysis using anti-BACE antibody and α-tubulin (loading control). B. BACE optical densities were normalized to their respective loading controls, averaged (+/-SD), and graphed, *p<0.05. C. Brains were immunostained using anti-BACE antibody. Images shown are representative from the temporal cortex. Arrows indicate regions taken for higher magnification.