Fig 10. Brain oxidative and nitrosative stress protein marker levels were not altered with 9 month airborne particulate matter exposure.
Brains of 9 month exposed filtered air (FA) or PM2.5 (PM) mice were collected with the left hemisphere fixed for immunohistochemistry and the right temporal cortices collected for biochemical analysis. Temporal cortex lysates from FA and PM brains were used for western blot analysis using A, anti-inducible nitric oxide synthase (iNOS), C, nitrated tyrosines (nitrotyrosine), E, 4-hydroxynonenal protein Michael adducts (HNE adducts) and α-tubulin (loading control) antibodies. Optical densities for B, iNOS and E, nitrotyrosine, and F, HNE adducts were normalized to their respective loading controls, averaged (+/-SD), and graphed.