Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 20;10(5):e0125745. doi: 10.1371/journal.pone.0125745

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Boucas et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 4 — (A) Mk2 ^-/- ;Mk3 ^-/- cells show a differential protein-RNA interactome in response to etoposide exposure when compared to wildtype cells (most-significant changes are highlighted). (B) Khsrp RNA immunoprecipitations (RIP) followed by Cdkn1a ^P21 qPCR validates interactome changes seen in wildtype and Mk2 ^-/- ;Mk3 ^-/- cells upon etoposide treatment. Upon etoposide exposure Khsrp is released from Cdkn1a ^P21 transcripts. In contrast, in Mk2 ^-/- ;Mk3 ^-/- MEFs, Khsrp-bound Cdkn1a ^P21 transcripts increase upon etoposide exposure. (C) Despite a typical arrest in G₂ upon etoposide treatment, (D) Mk2 ^-/- ;Mk3 ^-/- MEFs show a decreased G₁ population in comparison to wt cells. (E) Increased levels of the Cdkn1a ^P21 transcript in Mk2 ^-/- ;Mk3 ^-/- cells upon etoposide treatment (F) fail to promote the upregulation of Cdkn1a^P21 protein levels seen in wildtype cells.