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. 2015 May 20;10(5):e0125661. doi: 10.1371/journal.pone.0125661

Fig 2. Effect of RLIP76 expression on cell proliferation, colony formation and apoptosis in meningiomas cell lines.

Fig 2

(A) PCR and Western blot analysis revealed reduced RLIP76 mRNA and protein in IOMM-Lee and CH157-MN cells transfected with the RLIP76-targeted siRNA compared to GFP-transfected and control cells. (B) Effect of RLIP76 knockdown on IOMM-Lee and CH157-MN cell proliferation as measured by the MTT cell viability assay at different times after cell plating (*, P<0.05). (C) Effect of RLIP76 knockdown on IOMM-Lee and CH157-MN proliferation as measured by clonogenic assay (*, P<0.05). (D) The number of apoptotic cells was significantly higher in IOMM-Lee and CH157-MNcells transfected with RLIP76-siRNA as measured by PI staining and flow cytometry(*, P<0.05). (E) PCR and Western-blots revealed that caspase-3 mRNA and protein levels were significantly higher while Bcl-2 mRNA and protein expression was markedly lower in IOMM-Lee and CH157-MN cells transfected with RLIP76 siRNA.