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. 2015 May 20;10(5):e0126161. doi: 10.1371/journal.pone.0126161

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© 2015 Huang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — The leaf areas agroinfiltrated with each deleted (A, upper panel) or point-mutated (B, upper panel) NSs constructs were examined under UV and white light. The GFP fluorescence was recorded at 4 days after co-infiltration (dpa) of Agrobacterium separately carrying pBA-GFP (the expresser), pBA-GFi (the silencing inducer) and individual constructs with deleted or point mutated NSs proteins described in Fig 1A and 1B. Western blotting was performed for the detection of individually deleted (A, lower panel) or point-mutated (B, lower panel) NSs proteins expressed at 4 dpa. NSs monoclonal (MAb) and NSs polyclonal antibodies (PAb) were used for detecting NSs protein or NSs protein in which the common epitope was mutated, respectively. Expression levels of point-mutated NSs proteins, NSs mRNA, GFP and GFP mRNA were detected at 4 dpa (C). Coomassie blue-stained RuBisCO protein was used as loading controls for proteins and 18S rRNA used as loading controls for RNAs.