Fig 6. Symptoms on squash plants after inoculation with Zucchini yellow mosaic virus (ZYMV) recombinant viruses carrying different point-mutated NSs proteins.

(A) Symptoms on squash plants inoculated with individual ZYMV recombinants at 14 days post-infection (dpi). (B) Detection of mutated NSs proteins expressed by individual ZYMV recombinants at 14 dpi using anti-NSs MAb or anti-ZYMV CP PAb. Coomassie blue-stained RuBisCO protein was used as loading controls.