Fig 2. Histological analysis of horn buds and frontal skin from wild type fetuses (gd 115 and 140).

(A-D): Horn buds with multiple layers of vacuolated keratinocytes. Note the absence of hair follicles below the horn bud in (A-D). (A) and (C): Note thick nerve bundles in the dermis below the horn bud (black stars). (B) and (D): Represent magnifications (A) and (C). (E-H): Frontal skin. (E) and (G): Note the absence of thick nerve bundles in the dermis. (F) and (H) represent magnifications of (E) and (G). Haematoxylin and eosin. Gd = gestation days, ep = epidermis, de = dermis, hf = hair follicles.