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. 2015 May 20;11(5):e1004874. doi: 10.1371/journal.ppat.1004874

Fig 1. Passage of WNV in birds results in competitive fitness increases while viremia remains unchanged.

Fig 1

(A) WNV titers during passage. Open symbols represent samples with median viremias that were used for subsequent passage. (B) Viremia production after sequential passage, measured in young chickens (mean ± SD, n = 12–15 chickens each, data from passage replicates combined, *, P < 0.01,two-way ANOVA with Tukey’s correction). Dashed lines indicate the assay detection limits. (C) Competitive replicative fitness in young chickens (left; *, P = 0.0339; ****, P < 0.0001, unpaired t-test) and mosquitoes (right; ns, not significant; *, P < 0.05, unpaired t-test for both bodies and saliva). Passage replicates are colored as in (A) and horizontal lines represent the mean proportion of bird-passed WNV. Phenotypic assessment of wild bird passaged virus in its passaged host and in orally infected mosquitoes are in S1 Fig.