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. 2015 May 20;10(5):e0114586. doi: 10.1371/journal.pone.0114586

Fig 2. MEG3 overexpression promotes A549/DDP cell apoptosis in vitro.

Fig 2

(Fig 2A) Hoechst staining assay for cell apoptosis; the percentage of Hoechst-positive nuclei per optical field (at least 50 fields) was counted. (Fig 2B) Flow cytometry analysis of apoptosis in transfected A549/DDP cells in combination with increasing concentrations of cisplatin (0.0, 1.0, and 2.0 μg/ml). *P < 0.05.