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. 2015 May 20;10(5):e0127989. doi: 10.1371/journal.pone.0127989

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© 2015 Nieuwenhuizen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (a) Three state model with rates. (b) Cutout of total image of sparsely distributed DNA oligomers on glass labeled with single Alexa Fluor 647 dyes showing well-isolated clusters of localizations. (c) Cumulative number of localizations and single-exponential fit. (d) Correlation parameter Q determined from the spatial image correlations and fit with switching model shows agreement with the ground truth value determined from the cluster analysis. The estimated value for the average number of localizations ⟨M(t)⟩ shows agreement with the ground truth value determined from the cluster statistics. (e-g) Histograms of the number of localizations accumulated per cluster and model prediction at three time points during the image acquisition.