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. 2015 May 20;10(5):e0127989. doi: 10.1371/journal.pone.0127989

Fig 3. Quantitative localization microscopy with multiple emitters per labeled site.

Fig 3

(a) Number of localizations per neutravidin tetramer as a function of DOL as estimated from the image correlations and the ground truth values from cluster analysis, showing good agreement. (b) Fitted bleach rate k bl and switching rate k sw = M ∞ k bl as a function of DOL values for the same data, indicating independent activation and bleaching per label. Error bars indicate the standard deviation among samples at the same DOL. (c) Image of IgE receptors on the membrane of RBL cells labeled with primary antibodies with a DOL of 1.5 (k bl = 9.1 × 10−3/s and M ∞ = 2.3).