Mitochondria-Targeted RNA Import

. Author manuscript; available in PMC: 2015 May 20.

Published in final edited form as: Methods Mol Biol. 2015;1264:107–116. doi: 10.1007/978-1-4939-2257-4_11


ATP	1.5 μL

CTP	1.0 μL

GTP	1.5 μL

UTP	1.5 μL

10× Reaction buffer	2.0 μL

α-P³² -CTP	7.5 μL

Linear template DNA^a	0.1−1.0 μg

Enzyme mix	2.0 μL

Nuclease-free water	to 20 μL

^aUse 0.1–0.2 μg PCR-product template or ~1 μg linearized plasmid