Figure 4.
Typical UHPLC trace of isoflavones from a soy protein extract. Detection at 260 nm using a Hypersil Gold C18 column (100 × 2.1 mm; 1.9 μm; Thermo, San Jose, CA) and a 0.5 mL/min flow rate (9600 PSI) of a linear gradient of 0.1% aq. formic acid versus methanol/acetonitrile(1 : 1). FL = flavone (internal standard), other details in Fig. 1 and in text. Concentrations of IFL peaks (μM): D = 17.7, GLY = 6.3, G = 26.5, D-Mal = 16.8, GLY - Mal = 3.6, D-Ac = 5.9, G-Mal = 26.7, DE = 3.4, GLYE = 1.7, G-Ac = 8.3, GE = 4.4.