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. Author manuscript; available in PMC: 2016 Jun 10.
Published in final edited form as: Exp Cell Res. 2015 Apr 29;334(2):283–293. doi: 10.1016/j.yexcr.2015.04.014

Figure 3. N-terminus of Cdc45 physically interacts with the N- and helicase domain of HDHB.

Figure 3

(A) Expression level of GST N- (aa 1–303) and C-terminus (aa 304–566) of Ccd45 was determined by SDS-PAGE and Coomassie staining. (B) GST pull-down experiments; HDHB was added to the GST N- (aa 1–303) and C-terminus (aa 304–566) of Ccd45. Precipitated proteins were analyzed by SDS-PAGE and western blot with the indicated antibodies, either GST or HDHB antibody. (C) GST pull-down experiments; HDHB the N-terminus (aa 1–393), helicase domain (aa 394–958) or C-terminus (aa 957–1088) containing a His-tag was added to the GST N-terminus of Cdc45. Precipitated proteins were analyzed by SDS-PAGE and western blot with the indicated antibodies, either with His-taq or GST antibody.