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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: FEBS J. 2015 Mar 18;282(10):1906–1921. doi: 10.1111/febs.13243

Fig. 8.

Fig. 8

STD enhancements of ECL2S in the presence of mutgp120core/CD4M33. 2D-STD TOCSY enhancements calculated by dividing the peak intensities in the 2D-STD TOCSY by the corresponding peak intensities in a TOCSY spectrum. Cross-peaks intensities were normalized relative to the cross peak that exhibited the largest enhancement. Sample of 30 μM mutgp120core/CD4M33 with 600 μM ECL2S (1:20 molar ratio), 50 mM deuterated acetate pH 5.3, 50 mM NaCl, 1 mM deuterated-EDTA, 1:1000 Protease inhibitors cocktail and 0.05% NaN3 measured at 27 °C.