Fig. 3. Silencing of Sab or inhibition of JNK inhibited palmitic acid induced cell death.
(A) Cell death was inhibited by Sab knockdown, (B) JNK inhibitor refreshed 2 hourly, (C) Caspase 3 activation at 24 hours was inhibited by Sab knockdown and (D) JNK inhibitor, (E) Efficient knockdown of JNK protein expression was observed (Western blot), (F) Cell death (24 hours treatment of palmitic acid) with JNK 1 and/or 2 knockdown, (G) BHA, (H) Z-VAD-fmk (20μM). N = 3 experiments, *P<0.05.