Fig. 4. Time course of JNK activation and effect of Sab knockdown, JNKi, and antioxidant.
(A) Knockdown of Sab did not affect JNK activation at 1 and 4 hours but decreased P-JNK at 8 hours. (B) JNK inhibitor as expected decreased palmitic acid induced sustained JNK activation. (C) Time course of JNK translocation to mitochondria following palmitic acid treatment. The graph represents P-JNK densitometry normalized to OTC. (D) Time course of JNK activation in whole cell lysate from same experiments as C. The graphs represent P-JNK densitometry normalized to JNK. N=3 experiment (E) Time course of cell death in shlacZ vs shSab. N=3 experiments,*P<0.05.