Fig. 5.

Nuclear RNA foci start accumulating in number and size with time after they stopped dividing. A, B. DM1 NSCs cultured in proliferating condition. C-G. 8 days after Mitomycin C treatment. More and larger foci are seen in the nuclei. The biggest foci reach to 2μm in diameter (F). In some cells with fewer foci, large foci tend to localize to one pole of the cells (E, G). No cytoplasmic foci were seen on day 8 after Mitomycin C treatment. B, D: pictures were taken with some green background to show the cell contour