Table 2.
Primary antibodies used in this study
Complete list of antibodies, labeling, immunogens, manufacturers, species and dilutions.
| Antibody | Labeling | Immunogen | Manufacturer | Cat. No. | Species | Dilution | RRID |
|---|---|---|---|---|---|---|---|
| Anti-Myosin VIIA | Cochlear Hair Cells | Amino acids 877-1075 of human Myosin VIIA | Proteus Biosciences | 25-6790 | Rabbit Polyclonal | 1:100 | AB_10015251 |
| Anti-GFAP | Border Cells and Inner Phalangeal Cells (Supporting Cell Subtypes) | GFAP isolated from cow spinal cord | Dako (Denmark) | 70334 | Rabbit Polyclonal | 1:1000 | AB_10013382 |
| Anti-Sox2 | Supporting Cells and Stem Cells | Amino acids 277-293 of human Sox2 | Santa Cruz | sc-17320 | Goat Polyclonal | 1:100 | AB_2286684 |
| Anti-Sox10 | Supporting Cells and Schwann Cells | Amino acids 1-20 of human Sox10 | Santa Cruz | sc-17342 | Goat Polyclonal | 1:100 | AB_2195374 |
| Anti-NF200 | Spiral Ganglion Neurons | Recombinant fragment (Cow) | Abcam | Ab8135 | Rabbit Polyclonal | 1:1000 | AB_306298 |
| Anti-Krox20 | Schwann Cells | Amino acids 8-95 of Krox20 fused to GST | Covance | PRB-236P | Rabbit Polyclonal | 1:100 | AB_10064079 |
| Anti-Ki67 | Proliferating Cells | Human Ki67 C-terminus | Thermo Scientific | RM-9106-S1 | Rabbit Monoclonal | 1:250 | AB_149792 |
| Anti-Nestin | Nestin/Progenitor Cells | Rat (E15) spinal cord extracts | BD_Pharmagin | 556309 | Mouse Monoclonal | 1:100, 1:250, 1:500 | AB_396354 |
| Anti-Nestin | Nestin/Progenitor Cells | Full-length human recombinant nestin | Millipore | MAB5326 | Mouse Monoclonal | 1:100, 1:250. 1:500 | AB_2251134 |
| Anti-Nestin | Nestin/Progenitor Cells | EKE DQR FPR SPE EDQ Q; EKE RQE SLK SPE DED QQ; EVE EGP ERE QHQ ESL RS | Aves Labs | Cat#NES | Chicken Polyclonal | 1:100, 1:250, 1:500 | AB_2314882 |