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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Cell Immunol. 2015 Mar 31;295(2):92–98. doi: 10.1016/j.cellimm.2015.03.009

Fig. 5.

Fig. 5

TLR9 deficiency dampens cytokine production in the spleen after CLP. Age-matched WT and TLR9 KO mice were subjected to CLP. At 6 h after CLP, mouse spleens were harvested. Splenic CD4+ T cells were isolated and cultured with anti -CD3/anti-CD28 antibodies for the indicated times. Levels of IL-6 and IL-10 in the supernatants were determined by ELISA (a). RNA was extracted from CD4+ T cells and quantitative real-time PCR was performed (b). Data are mean ± SD for 4 mice per group. * p < 0.05; ** p < 0.01; *** p < 0.001.