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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Curr Drug Discov Technol. 2015;11(4):279–292. doi: 10.2174/1570163812666150120154701

Fig. (5).

Fig. (5)

Determination of the IC50 for BPP_03B04 against the activity of P. aeruginosa and S. pneumoniae PheRS. IC50 values for the inhibitory activity of BPP_03B04 against A.) P. aeruginosa PheRS or, B.) S. pneumoniae PheRS were 2.3 μM and 4.9 μM, respectively. IC50s were determined with the test compounds serially diluted from 200 μM to 0.4 μM into aminoacylation assays containing P. aeruginosa and S. pneumoniae PheRS at 0.05 and 0.1 μM, respectively. The concentrations of ATP and Phe were held constant at 2.5 mM and 75 μM.