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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Curr Drug Discov Technol. 2015;11(4):279–292. doi: 10.2174/1570163812666150120154701

Fig. (7).

Fig. (7)

Determination of the mode of action of BPP_03B04 relative to ATP. Inhibition by BPP_03B04 is uncompetitive with ATP. BPP_03B04 IC50s were determined using the aminoacylation assay in an SPA format. The phenylalanine concentration was fixed at 100 μM, and IC50s were determined at six different ATP concentrations ranging from 25 to 1000 μM. The data was fit to the sigmoidal dose response model using XLfit (IDBS) using IC50s determined in assays containing A.) P. aeruginosa PheRS or B.) S. pneumoniae PheRS.