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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Curr Drug Discov Technol. 2015;11(4):279–292. doi: 10.2174/1570163812666150120154701

Fig. (8).

Fig. (8)

Determination of the mode of action of BPP_03B04 relative to Phe. Inhibition by BPP_03B04 is competitive with Phe. BPP_03B04 IC50s were determined using the aminoacylation assay in an SPA format. The ATP concentration was fixed at 2.5 mM, and IC50s were determined at five different Phe concentrations ranging from 25 to 300 μM. The data was fit to the sigmoidal dose response model using XLfit (IDBS) using IC50s determined in assays containing A.) P. aeruginosa PheRS or B.) S. pneumoniae PheRS.