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Comparison of the kinetic parameters governing the interaction of PheRS with its substrates.
| PheRS | tRNA | Phe | ATP | ||||||
|---|---|---|---|---|---|---|---|---|---|
|
KM
(μM) |
kcat
(s−1) |
kcat/KM
(s−1, μM−1) |
KM
(μM) |
kcat
(s−1) |
kcat/KM
(s−1, μM−1) |
KM
(μM) |
kcat
(s−1) |
kcat/KM
(s−1, μM−1) |
|
| P. aeruginosa | 1.2 | 0.74 | 0.64 | 48 | 16.2 | 0.34 | 200 | 13.2 | 0.066 |
| S. pneumoniae | 0.94 | 0.93 | 1.0 | 21 | 22.5 | 1.1 | 225 | 15.5 | 0.070 |
