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OB were isolated from CD166-/- and WT littermate control mice. (A) Real-time PCR was used to examine mRNA expression of Fra1, Runx2, ALP, ColIa1, and Ocn. OB differentiation was also assessed using (B) ALP and (C) mineralization assays (bound calcium). Results are presented as the mean ± SD. *p<0.05 compared to WT OB.
