Table 1. Primary EpCAM+CD44+CD13+ gallbladder cells are the most enriched in colony forming ability.

(A) In two biologically separate experiments, LDAs were carried out on EpCAM+CD44+ and EpCAM+CD44− or EpCAM+CD13+ and EpCAM+CD13− cells. LDAs were performed on total EpCAM+ cells as controls. CFU frequency±SE (L-Calc^®) indicates CD13 and CD44 each independently enrich for colony forming ability. (B) Data from four biologically separate experiments indicating that EpCAM+CD44+CD13+ cells have the highest colony forming ability. “N/A” not performed because of insufficient cell number.

Sample	Sorted Population	CFU	SE in CFU
A
GB15 (d0)	EpCAM+	1/38	1/27 – 1/54
	EpCAM+CD44+	1/17	1/12 – 1/25
	EpCAM+CD44−	1/66	1/47 – 1/94
GB18 (d0)	EpCAM+	1/24	1/17 – 1/33
	EpCAM+CD13+	1/28	1/21 – 1/39
	EpCAM+CD13−	1/195	1/125 –
B
GB10 (d0)	EpCAM+	1/309	1/247 –
	EpCAM+CD44+CD13+	1/39	1/28 – 1/56
	EpCAM+CD44−CD13−	1/664	1/428 –
	EpCAM+CD44−CD13+	1/418	1/285 –
GB19 (d0)	EpCAM+	1/22	1/16 – 1/30
	EpCAM+CD44+CD13+	1/8	1/5 – 1/13
	EpCAM+CD44−CD13−	1/134	1/65 – 1/274
	EpCAM+CD44−CD13+	1/23	1/16 – 1/33
GB26 (d0)	EpCAM+	1/37	1/27 – 1/51
	EpCAM+CD44+CD13+	1/12	1/8 – 1/18
	EpCAM+CD44−CD13−	N/A
	EpCAM+CD44−CD13+	1/44	1/31 – 1/62
GB27 (d0)	EpCAM+	1/47	1/35 – 1/65
	EpCAM+CD44+CD13+	1/17	1/12 – 1/24
	EpCAM+CD44−CD13−	N/A
	EpCAM+CD44−CD13+	1/67	1/49 – 1/97