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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Am J Hematol. 2015 Mar 30;90(6):478–482. doi: 10.1002/ajh.23980

Figure 1.

Figure 1

Schematic illustration of the paper-based HbS assay and the characteristic blood stains. (a) To perform the assay a 20 μL droplet of blood mixed with Hb solubility buffer (phosphate buffer containing saponin and sodium hydrosulfite) is deposited on chromatography paper, a blood stain is allowed to form (polymerized deoxy-HbS is trapped in the paper mesh and soluble forms of Hb are wicked laterally), and the stain is scanned and analyzed automatically to estimate %HbS in the sample. (b) Characteristic blood stains produced on paper by samples with various %HbS. Scale bar is 1 cm.