Figure 8.

Real-time PCR analysis. mRNA levels were normalized with respect to GAPDH, chosen as an internal control. Differentiation stages initiated 4 passage human Wharton’s jelly Mesenchymal Stem Cells (hWJ-MSCs) and ended in spermatogonial colony like cells. Histograms show mean expression values (± SD, n=3; p ≤ 0.05).α, β, μ, δ and λ: significant difference with other groups in the same gene; *: significant difference with control (hWJMSC) and Wj+BMP4+RA groups in ITΒ1 gene. RA; Retinoic acid, BMP4; Bone morphogenic protein 4