Table 1.
Gene | Primer sequence | Length | Code number | Tm |
---|---|---|---|---|
Itα6 | F:5´-CCCTGATGTTGCTGTTGGTTC-3´ R:5´- TGGCGGAGGTCAATTCTGTTAG-3´ | 114 | NM_000210.2 | 59 |
Itβ1 | F:5´-TAGCAAAGGAACAGCAGAGAAG-3´ R:5´-AGGTAGTAGAGGTCAATGGGATAG-3´ | 150 | NM_002211.3 | 58 |
Stra8 | F:5´- AAGGACAGCGGCGTGGAC -3´ R:5´- CTGGCAAGCACTGAACTGGAG -3´ | 149 | NM_182489.1 | 61 |
Plzf | F:5´-AAGTTCAGCCTCAAGCATCAG- 3′ R:5´- CGTTGTGCGTTCTCAGGTG- 3′ | 133 | NM_00101801 | 59 |
Ddx4 | F:5´- CTTAGACCCAGACGAATGTATGC-3´ R:5´- GTTCACTTCCACTGCCACTTC-3´ | 119 | NM_001166533.1 | 58 |
Pou5f1 | F:5´- CCATCTGCCGCTTTGAGG-3´ R:5´- ACGAGGGTTTCTGCTTTGC-3´ | 133 | NM_001285987.1 | 58 |
Dazl | F:5´- GCTCGCCTGACGCCATCTTTG-3´ R:5´- GCTGATGAGGACTGGGTGCTG-3´ | 98 | NM_001190811.1 | 62 |
Piwil2 | F:5´-TGGTTGGAGTAGGACGCTTG-3´ R:5´- GGGACGGTGTGCTGAAGG-3´ | 122 | NM_001135721.1 | 59 |
Fragilis | F :5´- GCACCCTCTACCTGAATCTG-3´ R:5´- AGGATGTTGTAGCACTTGGC-3´ | 136 | NM_001025295.2 | 58 |