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. 2015 Apr;18(4):325–333.

Table 1.

Genes, primers, and sizes of amplification products (bp) for quantification of gene expression by real-time quantitative polymerase chain reaction

Gene Primer sequence Length Code number Tm
Itα6 F:5´-CCCTGATGTTGCTGTTGGTTC-3´ R:5´- TGGCGGAGGTCAATTCTGTTAG-3´ 114 NM_000210.2 59
Itβ1 F:5´-TAGCAAAGGAACAGCAGAGAAG-3´ R:5´-AGGTAGTAGAGGTCAATGGGATAG-3´ 150 NM_002211.3 58
Stra8 F:5´- AAGGACAGCGGCGTGGAC -3´ R:5´- CTGGCAAGCACTGAACTGGAG -3´ 149 NM_182489.1 61
Plzf F:5´-AAGTTCAGCCTCAAGCATCAG- 3′ R:5´- CGTTGTGCGTTCTCAGGTG- 3′ 133 NM_00101801 59
Ddx4 F:5´- CTTAGACCCAGACGAATGTATGC-3´ R:5´- GTTCACTTCCACTGCCACTTC-3´ 119 NM_001166533.1 58
Pou5f1 F:5´- CCATCTGCCGCTTTGAGG-3´ R:5´- ACGAGGGTTTCTGCTTTGC-3´ 133 NM_001285987.1 58
Dazl F:5´- GCTCGCCTGACGCCATCTTTG-3´ R:5´- GCTGATGAGGACTGGGTGCTG-3´ 98 NM_001190811.1 62
Piwil2 F:5´-TGGTTGGAGTAGGACGCTTG-3´ R:5´- GGGACGGTGTGCTGAAGG-3´ 122 NM_001135721.1 59
Fragilis F :5´- GCACCCTCTACCTGAATCTG-3´ R:5´- AGGATGTTGTAGCACTTGGC-3´ 136 NM_001025295.2 58