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. 2015 May 7;2015:479565. doi: 10.1155/2015/479565

Table 1.

List of RT-PCR primers.

Gene NCBI GeneID Forward Reverse Amplicon size (bp)
Glut1 24778 CTTTGGCAGGCGGAACTC TTGCCCAGTTTGGAGAAACC 84
Actb 81822 TTCCTTCCTGGGTATGGAATCC GGATGTCAACGTCACACTTCATG 77
Hk2 25059 TGGGCTGGACAACCTCAAAG CCTTGGCAAAGTGAGGATGAAG 74
Pdk1 116551 GCGAGACGGCTTTGTGATTTG CCTGGTGATTTCGCATTTAGTTC 77
P53 24842 CACTCCAGCTACCCGAAGAC GCCAGGAACCAGTTTGCATAG 259
Ldha 24533 AATGAAGGACTTGGCTGATGAG GCCATGCTGAAGATCCATCATC 85
Tigar 502894 ACGCCTTCTCCAGTGATCTC CGTACATCCTTTCCCGAAGTC 120
Gpt2 307759 CCCACAGGCCAGGTACAAAG AAAGCTTCTCTTCCCAGGCAAAG 70
Pkm2 25630 TGACACCTTCCTGGAACACATG GGGAAGCAGGGCCAATGG 98

Gene abbreviations: Glut1, solute carrier family 2 (facilitated glucose transporter) member 1; Actb, beta actin; Hk2, hexokinase 2; Pdk1, pyruvate dehydrogenase kinase isozyme 1; P53, tumor protein p53; Ldha, lactate dehydrogenase A; Tigar, fructose-2,6-bisphosphatase TIGAR; Gpt2, glutamic pyruvate transaminase (alanine aminotransferase) 2; Pkm2, pyruvate kinase muscle.