RT-PCR and Western blot analysis of human prostate tissues. (A) Detection of mRNA of Rac isoforms 1–3 by RT-PCR in periurethral prostate tissues from n = 7 patients. Expression of Rac is referred to as expression of 18SrRNA. Shown are values for each sample (ratios of Ct values), and the median for each target. Note: the higher the value, the lower the expression of the target. (B) Periurethral prostate tissues from n = 7 patients were subjected to Western blotting using antibodies for Rac isoforms 1–3, calponin (smooth muscle marker), pan-cytokeratin (marker for epithelium/glands), PSA (marker for BPH), and β-actin (housekeeping, loading control).