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. 2015 Mar 26;172(11):2918–2927. doi: 10.1111/bph.13097

Figure 4.

Figure 4

DHEA reduced cortisol-induced GRα binding to the GRE present in the GNB2L1 promoter. THP-1 cells were treated for 18 h with DHEA (10 and 100 nM) or DMSO as vehicle control. Cortisol (0.1 and 0.5 μM) was then added for 1 h. Nuclear extracts of THP-1 were used to evaluate the binding of GRβ (A) and GRα (B) to the GRE domain. GRβ/GRα binding ratio has been calculated on these results (C). Each value represents the mean ± SD of three to five independent experiments. Statistical analysis was performed with Turkey's multiple comparison test with *P < 0.05, **P < 0.01, ***P < 0.001 versus control.