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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Mar 26;1850(7):1397–1404. doi: 10.1016/j.bbagen.2015.03.009

Figure 2. Effect of arabinose in the expression and biotinylation of VHH T7.

Figure 2

Different concentrations of arabinose were used to induce the co-expression of VHH T7 and BirA as described in the legend. The graph displays the reactivity of serial dilutions of the VHH T7 soluble extract with its cognate antigen (TCC-BSA) detected with a streptavidin-HRP conjugate. The midpoints of the curves are listed for comparison. The SDS-PAGE analysis of VHH T7 expression in the soluble freeze-and-thaw extract is shown in the insert.