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. 2015 Apr 5;6(6):511–518. doi: 10.7150/jca.10830

Figure 5.

Figure 5

Tissue array analysis of TGF-β and PTHrP expression in HCC and non-cancerous specimens. A human tissue array containing 38 HCC cancer tissues and 9 non-neoplastic liver tissues was double-stained for PTHrP and TGF-β by immunohistochemistry using the Poly-HRP Anti-Mouse IgG Detection System (brown staining - TGF-β) and the Biotin SP-AP Detection System (blue staining - PTHrP), respectively. The nuclei were counterstained with nuclear fast red. (A) A representative image showing PTHrP staining but no TGF-β staining in the HCC cancer cells in a HCC patient (ID #08). (B) TGF-β was intensely stained in the matched non-neoplastic liver tissue of the same patient. (C) PTHrP expression level was considerably higher in HCC tissues than in the non-neoplastic liver tissues. (D) TGF-β expression was high in the non-neoplastic liver tissues but low in HCC cancer tissues. Original magnification: x400.