Fig. 1.

Phenotype of imDC and matured DC

Transmission electron microscopy and flow cytometry assay were used to evaluate the phenotype of imDCs generated from UCB after 6 days of culture in the presence of rhSCF, rhGM-CSF and rhIL-4, and mature DCs treated by BGC823 TAAs for 2 days. A) The morphology of the imDCs was observed under a light microscope (magnification 200×). B) The expression of several molecules (MHC-I, MHC-II, CD3, CD40, CD80, CD54, CD11c, and CD86) was significantly higher in the DCs loaded with tumor antigens than in the immature DCs, p < 0.01. Furthermore, the expression of molecules on the DCs transfected with BGC823 cells total RNA were higher than of those on the DCs loaded with lytic-Ag of BGC823 cells, p < 0.05. Data in the flow cytometry pictures were from one representative experiment of four independent experiments. C) The morphology of the mature DCs was observed under a light microscope (magnification 200×)