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. Author manuscript; available in PMC: 2016 Jan 13.
Published in final edited form as: Sci Signal. 2015 Jan 13;8(359):ra4. doi: 10.1126/scisignal.2005906

Fig. 4. EGFR:GRB2 PLA in PDX tumors in mice.

Fig. 4

(A and B) Graphs of the variability between replicate cores in a PDX tumor microarray labeled by (A) EGFR:GRB2 PLA or (B) EGFR AQUA. N=289 tumors. For 2 PDX tumors only a single core was available. PLA intensity was scored manually from 0 to 3+ based on the number of foci per cell by blinded observers. For B, P < .0001, Pearson correlation. (C and D) Graphs of the relationship between EGFR:GRB2 PLA and EGFR AQUA for individual cores across all cancer types (C) or in NSCLC PDX tumors (D). Bars represent means ± 95% confidence interval (CI). For C, N=579 cores from 289 tumors. 3 cores were missing either EGFR:GRB2 PLA or EGFR AQUA values. P <.0001, Kruskal-Wallis test. For D, N=119 cores from 60 tumors. Only a single core was available for one tumor. EGFR:GRB2 PLA scores were grouped as Low (0 or 1) or High (2 or 3) . *P < 0.001, Two-tailed Mann-Whitney U-test. (E) Graphs of percent of PDX tumors of the indicated types with EGFR:GRB2 PLA scores grouped as in D. adeno = adenocarcinoma, squam = squamous cell carcinoma, NOS = not otherwise specified. N in parenthesis indicates the number of PDX tumors.