Figure 4.

Effect of SIRT1 overexpression and silencing on the migration of EPCs. A, B. Representative photographs of SIRT1 overexpression and silencing on the migration of EPCs. EPC migration in response to Ad-SIRT1 and si-SIRT1 was detected using the Transwell system. C, D. The migration of EPCs transfected with Ad-SIRT1 was enhanced as compared with that of Ad-GFP-transfected EPCs, but knockdown of endogenous SIRT1 significantly reduced the migration of EPCs compared with the Ad-GFP group. The results are expressed as the mean ± SD (*P < 0.05 vs. Ad-GFP).