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Effects of SIRT1 overexpression and silencing on the proliferation of EPCs. Proliferation of EPCs was examined using the MTS assay. A. EPCs were transfected with or without Ad-GFP, and Ad-SIRT1. Three separate experiments were done in triplicate. B. EPCs were transfected with or without negative control of siRNA or si-SIRT1. The effect was decreased in the presence of si-SIRT1. The results are expressed as the mean ± SD (*P < 0.05 vs. Ad-GFP or si-CON).
