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Influence of SIRT1 silencing on changes in levels of SIRT1. Changes in levels of protein and mRNA of SIRT1 were detected using western blotting and RT-PCR analysis after silencing of SIRT1 in EPCs. Values are the percentage of GAPDH. SIRT1 gene and protein levels were decreased by SIRT1 silencing, but si-CON was not affected (*P < 0.05 vs. control).
