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. 2015 Mar 1;8(3):2473–2483.

Figure 3.

Figure 3

Promotion of lithium on GFP-MSCs neural differentiation in vitro. A-C. Neural differentiation of GFP-MSCs. To induce GFP-MSCs neural differentiation in vitro, dissociated single cells were cultured in neural induction medium with or without lithium (0.1 μM) for 4 weeks, followed by immunostaining. GFP-MSCs differentiated into (A) βIII-tubulin+ neurons, B. GFAP+ astrocytes and (C) olig-2+ oligodendrocytes. Nuclei were stained with DAPI. Scale Bar = 50 μm in A, B and C. One representative experiment of 3 is shown (n = 5 wells in each group). D. Quantitative analysis of percentages of GFP-MSCs differentiated into each type of neural cells among total numbers of GFP-positive cells. Data represent mean ± S.D. of three independent experiments. *P < 0.05, comparison between neural induction medium treated GFP-MSCs (Control) and lithium plus neural induction medium treated GFP-MSCs (Lithium).