Figure 2.

PVT1 siRNA rescued hypertrophic responses in cardiomyocytes. A. After 72 h of culture, relative fold change of PVT1 expression in the cultured neonatal mouse cardiac cardiomyocyte of starvation group, (starvation + Ang II) group and (starvation + Ang II + siRNA) group vs. control group, respectively. B. Representative photograph of cardiomyocytes transfected with PVT1 siRNA after Ang II treatment (Hoechst 33342 staining). C. Cell surface areas of the cardiomyocytes were measured using Image J software (n = 100). Scale bars, 20 μm. The different letters (a, b and c) indicated significant differences (P < 0.05).