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. 2015 Mar 1;8(3):2590–2602.

Figure 2.

Figure 2

Mutation of the Cosmc gene in Tn+ cells, including Jurkat and HT-29-Tn+ cells, results in lower or a complete absence in T-synthase activity. A. Enzyme activity assay. Cytoplasmic extracts of Tn+ cells, including Jurkat and HT-29-Tn+ cells, and Tn- cells including normal, BGC823 and HT-29-Tn- cells. Which were all generated in triplicates for T-synthase assay using GalNAc-α-4MU as receptor. B. Analysis of Cosmc and T-synthase expression by RT-PCR. mRNA from the cells that we detected was prepared with Trizol, using universal primers to synthesize cDNA. The PCR products were analyzed on 1% agarose gel. C. The mutation of Cosmc in Jurkat cells. PCR products generated using cDNA as template were analyzed on 1% agarose gel, purified and then sequenced. Arrowhead indicates the mutation site.