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Loss of the active Cosmc allele CDS in HT-29-Tn+ cells. A. PCR analysis of the Cosmc gene. gDNA were extratced from cells and then amplified using corresponding primers. PCR products were analyzed on 1% agarose gel. B. Analysis of SNPs in the non-coding region in Cosmc. Non-coding regions were amplified using gDNA as templates. PCR products were analyzed on 1.5% agarose gel, purified and then sequenced.
