Figure 4.

The methylation status of CpG islands in Cosmc. A. The difference between methylated and non-methylated sites after treatment with bisulfate. gDNA from cells were treated with bisulfate and amplified by PCR. CpG islands of Cosmc were identified using specific primers. PCR products were analyzed on 1.5% agarose gel, purified and then subjected to thymine-adenine (TA) colone sequencing. Portions of the sequencing results are shown including methylation and non-methylation sites. Methylation sites detected were underlined and SP1/3 binding sites of Cosmc were boxed. B. CpG methylation status of Cosmc in normal female cells. The amount of CpG island methylation containing 25 methylation sites were examined in normal fenmale cells using BSA. Nine monoclonal colonies were selected for sequencing. SP1/3 binding sites of Cosmc were boxed. C. The amount of CpG island methylation in BGC823, HT-29-Tn⁺ and HT-29-Tn^- cells. Ten monoclonal colonies were selected for sequencing. SP1/3 binding sites in Cosmc were boxed.