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. 2015 Mar 1;8(3):2638–2648.

Figure 1.

Figure 1

Increased expression of NUF2 in human pancreatic cancer tissues and cell lines. A. mRNA levels of NUF2 were detected by real-time quantitative PCR in 10 pairs of PC tissues and adjacent non-cancer tissues. GAPDH gene served as an internal control. Scatter dots represent relative mRNA expression levels and the horizontal lines represent mean with SD. Statistical significance: **P < 0.01, compared to normal tissues. B. qRT-PCR analysis of NUF2 expression in 3 human pancreatic cancer cell lines and HEK293 cell line. GAPDH gene was used as an internal gene. Data represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, compared to HEK293 cells. C. Western blot analysis of NUF2 expression in 3 human pancreatic cancer cell lines Sw1990, PANC-1, BXPC-3 and HEK293 cell line. GAPDH gene served as an internal control. Data represent one of three independent experiments with similar results. *P < 0.05, **P < 0.01, compared to HEK293 cells.