Figure 2.

Increase of histone acetylation and histone acetylase(HAT) activities in HG-induced EMT of HPMCs. A. Expression of acetylated histone H3 and H4 in HG-induced EMT were detected by western blot assay. β-Actin was used as an internal control. B. HAT activities were determined using the commercially available HAT Activity Colorimetric Assay kit according to the manufacture’s protocol. C. The expression level of HAT subtypes p300 and GCN5 mRNA were detected using qRT-PCR assay. Data are expressed as means ± SD from three independent experiments and analyzed by one-way ANOVA. *P < 0.05, **P < 0.01 vs. without HG group.